Lentibacillus Lipolyticus Sp. Nov., A Moderately Halophilic Bacterium Isolated From Shrimp Paste (Ka-Pi)

A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9(T), was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37-40 degrees C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9(T) and Lentibacillus juripiscarius TISTR 1535(T) and Lentibacillus halophilus TISTR 1549(T) were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9(T) represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA G+C content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C-15:0, anteiso-C-17:0 and iso-C-16:0. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9(T) represents a novel species of Lentibacillus, for which the name Lentibacillus lipolyticus sp. nov. is proposed. The type strain is SSKP1-9(T) (=JCM 32625(T) =TISTR 2597(T)).