Aggregation-Induced Emission-based Ionic Liquids for Bacterial Killing, Imaging, Cell Labeling, and Bacterial Detection in Blood Cells.

A series of aggregation-induced emission (AIE)-based imidazolium-type ionic liquids (ILs) were designed and synthesized for bacterial killing and imaging, cell labeling, and bacterial detection in blood cells. The AIE-based ILs showed antibacterial activities against both Escherichia coli and Staphylococcus aureus. The carbon chain length of substitution at the N3 position of the imidazolium cations highly affects the antibacterial properties of ILs. Owing to their AIE characteristics, the ILs could selectively capture fluorescence image of dead bacteria while killing the bacteria. The fluorescence intensity varied with the concentration of bacteria, indicating that AIE-based ILs has potential as an antibacterial material and an efficient probe for bacterial viability assay. In addition, the synthesized AIE-based ILs exhibit relatively low cytotoxicity and hemolysis rate and therefore potential for cell labeling, as well as bacterial detection in blood cells.