Actinomadura Logoneensis Sp. Nov., A Novel Actinomycete Isolated From The Soil

One novel actinobacterial isolate, designated strain NEAU-G17(T), was isolated from muddy soil collected from a riverbank in Chad and characterised using polyphasic approach. Morphological and chemotaxonomic characteristics of this strain coincided with those of the genus Actinomadura. The 16S rRNA gene sequence analysis showed that strain NEAU-G17(T) was most closely related to Actinomadura oligospora JCM 10648T (98.7%) and Actinomadura gamaensis NEAU-Gz5(T) (98.6 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G171- formed a cluster with Actinomadura oligospora JCM 10648(T), Actinomadura gamaensis NEAU-Gz5(T) and Actinomadura rupiterrae CS5-AC15(T) (96.4 %). However, the combination of DNA DNA hybridisation values and some phenotypic characteristics allowed the isolate to be differentiated from their most closely related species. The major menaquinones were identified as MK-9(H-4), MK-9(H-6) and MK-9(H-8). Their polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as C-16:0, iso-C-16:0 and C-18:1 omega 9c. Therefore, it is proposed that strain NEAU-G17(T) should be classified as representative of a novel species of the genus Actinomadura, for which the name Actinomadura logoneensis sp. nov. is proposed. The type strains is NEAU-G17(T) (=CGMCC 4.7411(T) DSM 105122(T)).