Quantifying blood-brain-barrier leakage using a combination of Evans blue and high molecular weight FITC-Dextran.
Journal of Neuroscience Methods(2019)
摘要
•Binding EB and high MW FITC-Dextran is able to quantify and visualize BBB leakage.•Air drying at RT after slicing achieves stable and reliable results of BBB leakage.•Sections processed with this method enable further immunofluorescence staining.
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关键词
apoE-/-mouse,Blood-brain-barrier leakage,Evans blue,FITC-Dextran,Lipopolysaccharide
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