Crystal Structure of the Tubulin Tyrosine Carboxypeptidase Complex VASH1–SVBP
Nature structural & molecular biology(2019)
Abstract
The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which folds in a long helix, which stabilizes the VASH1 catalytic domain. This structure, combined with molecular docking and mutagenesis experiments, reveals which residues are responsible for recognition and cleavage of the tubulin C-terminal tyrosine.
MoreTranslated text
Key words
Microtubules,Molecular modelling,Proteases,X-ray crystallography,Life Sciences,general,Biochemistry,Protein Structure,Membrane Biology,Biological Microscopy
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined