Chromosome Conformation Capture That Detects Novel Cis- and Trans-Interactions in Budding Yeast.

•Taq I-3C incorporates a novel normalization step to account for cleavage efficiency.•Uses real-time qPCR to quantify interaction frequencies.•Permits comparison of chromatin contacts in different physiological contexts.•Has sufficient resolution to capture UAS-promoter interactions.•Has led to the identification of evanescent intragenic and intergenic contacts.