Generation of an integration-free human induced pluripotent stem cell line MUSIi010-A from occipital scalp fibroblasts of a male patient with androgenetic alopecia.

Nutnicha Tantarungsee, Zaukir Bostan Ali,Rungtip Soi-Ampornkul,Chuda Rujitharanawong,Chanika Subchookul,Alisa Ritthaphai,Bootsakorn Boonkaew,Methichit Wattanapanitch,Kanchalit Thanomkitti,Daranporn Triwongwaranat,Chinnavuth Vatanashevanopakorn,Rattapon Thuangtong

Stem cell research（2019）

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Abstract

An induced pluripotent stem cell (iPSC) line, MUSIi010-A, was established by Sendai virus (SeV) transduction of scalp fibroblasts from a 59-year-old male with androgenetic alopecia (AGA). Pluripotency of the iPSC line was verified by immunofluorescence staining of pluripotent markers and by in vitro trilineage differentiation. The MUSIi010-A line was shown to retain normal karyotype and free of SeV vectors at passage 17. This iPSC line can be used for studying pathological mechanisms of AGA.

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