Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce ( var. ramosa Hort.)

A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85 T , was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85 T was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85 T belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925 T (98.5%) and also to Leifsonia kafniensis NCCB 100216 T (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atp D, dna K, rec A, rpo B, sev Y and ych F genes showed that strain NEAU-85 T belongs to the genus Cryobacterium . The digital DNA–DNA hybridization values revealed that strain NEAU-85 T is distinct from its close phylogenetic neighbour C. psychrotolerans . The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso -C 15:0 , anteiso -C 15:1 and iso -C 16:0 . The genomic DNA G + C content of strain NEAU-85 T was determined to be 68.9 mol%. The DNA–DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85 T can be concluded to represent a novel species of the genus Cryobacterium , for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85 T (= CCTCC AA 2018035 T = JCM 32545 T ).