Phage-Displayed Peptide of Keratinocyte Growth Factor and Its Biological Effects on Epidermal Cells

Using monoclonal anti-human keratinocyte growth factor (KGF) antibody as the target in this study, we screened the phage display 7-mer peptide library to isolate key sequences of KGF. Through alignment analysis, the key sequences of KGF were selected and used to synthesize bioactive peptides. After purification, structural modification and fluorescence label, the phage model peptides of KGF were added to epidermal cells. CCK-8 assay, quantitative real-time PCR analysis and Western blot analysis were employed to evaluate the effect of the phage model peptides on epidermal cells. Among the 26 sequences isolated from a phage display 7-mer peptide library, only one sequence was chosen to generate bioactive peptide of KGF. The synthesized peptide could bind to epidermal cells, facilitate cellular proliferation, and promote both the mRNA expression and protein expression of KGF receptor. The result of this study is expected to promote wound healing by using phage-displayed KGF peptide, which can play biological effects on epidermal cells.