Activating A 2x2 Network Of Hnt Astrocytes With Uv Laser Stimulation

To further investigate the communication in astrocytic networks, in vitro astrocytes were patterned in simple networks by using parylene-C/SiO2 platform. We designed a 2x2 small network on parylene-C/SiO2 platform and demonstrated, for the first time, that the intracellular calcium response of a single human hNT astrocyte stimulated by UV laser pulses can be transmitted to neighboring astrocytes in this 2x2 small network. The calcium responses of the astrocytes network were recorded and analyzed.