531 – Targeting Nerve-Cancer Metabolism Improved Overall Survival in Aged Mice with Gastric Cancer

Figure A: Tumor-conditioned LECs express ENA-78 (a)Cytokine antibody array detected the level of human chemokines and inflammations expressed in the supernatants of tumor conditioned LECs (t-LECS) and normal LECs (n-LECs).(b,c,d) The set of c indicated the cytokines which express in t-LECs CM but not in TCM.ENA-78 is obviously overexpression in t-LECs than n-LECs.(e)ELISAs for human ENA-78 (R&D System) detected t-LECS CM obtained at Day 0, 1, 2, 3, 4 of TCM induction.(n=3).(f)ENA-78 concentration in each CM was determined at 72 h by ELISAs.TCM induced ENA-78 overexpression in t-LECs, compared to n-LECs (**P < 0.01) (n=3).(g,h)TCM (100 μL) and serum-free media (SFM) were administered into nude mice for 2 weeks.Lymph nodes from TCM treated animals showed mENA-78 expression around mouse lymphatic vessels.Scale bars, 50 μm.(** P<0.002, n=7) (i)The ELISA determined the expression of mENA-78 in the liver, lymph node and lung by tissue homogenate method.