Single Nucleotide Polymorphisms in Two Inflammation-Related Genes and Chronic Periodontitis risk in Romanian Adults

Background: The existing literature showed conflicting data on the association between peridontos s and IL6 and TGFb1 polymorphisms. Aim to investigate the correlation between susceptibility to chronic periodontitis and TGFb1 C-509T, IL6 G-174C polymorphisms and TTV infection in Romanian population. Materials and methods: Five hundred and fifteen subjects (260 healthy and 255 patients with chronic periodontitis) of Romanian origin were selected for this study. DNA was extracted from peripheral blood samples and the two polymorphisms and the presence of torque teno virus DNA were tested by PCR-based methods. StatsDirect was used for data analysis. Results: Our data showed that lifestyle choices (e.g. inadequate personal oral hygiene -O.R. =7.66, p<0.0001, taking irregular meals -O.R. =2.82, p<0.001 and smoking O.R.=1.5, p=0.01) increase the risk of periodontitis. We also identified differences in distribution of TGFb1 C-509T and IL-6 G-174C polymorphisms in patients and control groups. However, there was no association between the distribution of TGFb C-509T variants and the risk of periodontitis in male (p=0.85). There was an association between the presence of IL6 -174G allele and chronic periodontitis in the overall group of subjects (O.R.=1.6, p=0.01) and in men (O.R.=1.82, p=0.02), but not in women (p=0.2). The prevalence of TTV DNA and association with periodontitis was consistent with previous studies. Conclusions: Lifestyle choices, IL6 G-174C and TTV were associated with periodontitis in our lot. Further investigation conducted on a larger group with less confounding factors is required in order to validate these findings.