Background free tracking of single RNA in living cells using catalytically inactive CasE

Chunyu Han,Feng Gao,Feng Jiang, Xiaoyue Bai, Yue Sun

bioRxiv（2019）

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Abstract

RNAs have important and diverse functions. Visualizing an isolated RNA in living cells provide us essential information of its roles. By now, there are two kinds of live RNA imaging systems invented, one is the MS2 system and the other is the 13a system. In this study, we show that when fused with split-Fp, E can be engineered into a live RNA tracking tool.

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