Protective Effect Of Zinc Supplementation On Renal Ischemia/Reperfusion Injury In Rat: Gender-Related Difference

Background: Zinc (Zn) knows as essential microelement which prevents oxidative stress. The effect of Zn supplement on renal function parameters in rats subjected to renal ischemia-reperfusion (IR) injury was investigated. Methods: Male and female rats were subjected to renal IR with and without Zn sulfate (10 mg/kg/day for 5 days) supplementation. The kidney function markers and histology findings in Zn-treated group were compared with sham and control groups. Results: The serum levels of blood urea nitrogen and creatinine (Cr) and kidney tissue damage score were increased significantly after renal injury (P < 0.05) gender dependently, but no alterations were observed for these markers in Zn-treated animals after renal IR injury. Cr clearance was significantly different between genders (P < 0.05); however, Zn supplementation increased the Cr clearance and kidney nitrite level significantly in male rats (P < 0.05). Zn. also increased urine flow in female (P < 0.05), but it did not alter urine load of Na (U-Na V) and percentage of Na excretion (E-Na%). Conclusions: Zn may improve renal function after IR injury gender dependently.