The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets.

Methods in molecular biology (Clifton, N.J.)(2019)

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摘要
Cell-tracker fluorescent dye labeling is widely used for investigating antigen-specific immune responses in vitro and in vivo. Here we describe a development of this technique-the use of dual-cell-tracker dye staining for the identification and characterization of the responses of different T-cell subsets to peanut proteins in vitro.
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关键词
Allergy,CFSE,Cell-tracking dyes,Flow cytometry,Proliferation,eFluor670
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