Evaluation of subculture ages on organogenic response from root callus and SPAR based genetic fidelity assessment in the regenerants of Hibiscus sabdariffa L.

•Induction of organogenic callus from in vitro grown roots of Hibiscus sabdariffa.•Histological proof of shoot bud differentiation from callus.•Rooting, hardening and study of field performance of regenerated shoots.•Declining of shoot regeneration efficiency with subculture passages.•SPAR method ensured true-to-type plant production upto five consecutive subcultures.