Genome-wide CRISPR-Cas9 screening in mammalian cells

Methods（2019）

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摘要

•CRISPR-Cas9 technology has revolutionised genome-wide mutagenic screens.•Phenotypic screens are now accessible to most laboratories.•Extension of Cas9 functionality permits future deployment of creative screens.

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关键词

Genetic screen,CRISPR-/Cas9,Mammalian cells,gRNA

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