MP02-19 OPTIMIZING THE NANOPARTICLE ENHANCED ADHESION OF MUSSEL INSPIRED HYDROGELS FOR TISSUE INTERFACING

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Pelvic Prolapse (MP02)1 Apr 2019MP02-19 OPTIMIZING THE NANOPARTICLE ENHANCED ADHESION OF MUSSEL INSPIRED HYDROGELS FOR TISSUE INTERFACING Nikhil Pandey*, Andres Urias, Valinda Jones, Serkan Yaman, Amirhossein Hakamivala, Boris Rodionov, Jun Liao, Philippe Zimmern, Kytai Nguyen, and Yi Hong Nikhil Pandey*Nikhil Pandey* More articles by this author , Andres UriasAndres Urias More articles by this author , Valinda JonesValinda Jones More articles by this author , Serkan YamanSerkan Yaman More articles by this author , Amirhossein HakamivalaAmirhossein Hakamivala More articles by this author , Boris RodionovBoris Rodionov More articles by this author , Jun LiaoJun Liao More articles by this author , Philippe ZimmernPhilippe Zimmern More articles by this author , Kytai NguyenKytai Nguyen More articles by this author , and Yi HongYi Hong More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000554931.86549.51AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: Mussel inspired approaches to create improved bioadhesives involve synthetic hydrogel systems with catechol moieties grafted onto polymer chains [2], albeit these have lower adhesiveness compared to mussel's strong underwater adhesion. We have demonstrated that the combination of nanoparticles (NPs) and mussel-inspired hydrogel systems can create enhanced tissue adhering nanocomposites (NCs) [3]. Herein we further optimize the tissue adhesion of a nanocomposite of hyaluronate based biomimetic, mussel-inspired hydrogel and PLGA or polydopamine NPs, within a tissue interface, by varying the nanoparticles types, sizes and concentrations. METHODS: Dopamine was grafted onto sodium hyaluronate (HA) (MW:151-300KDa) using EDC/NHS aqueous chemistry, creating a mussel-inspired polymer (HA-Dopa) [4]. NCs were formulated combining HA-Dopa with, poly (D, L-lactic-co-glycolic acid) (PLGA) NPs, or N-hydroxysuccinimide (NHS) modified PLGA NPs (PLGA-NHS) or polydopamine (polydopa) NPs using sodium metaperiodate (PI) as a cross-linker. NCs tissue adhesiveness (Uniaxial lap shear testing, cross head speed:10mm min-1) on a porcine skin-muscle interface (Figure 1A), along with effect of nanoparticle concentration (0-20%w/v), type, and size on the adhesion was investigated. Finally, in vitro cytocompatibility of the optimized NCs was evaluated using human dermal fibroblasts (HDFs). RESULTS: NP mediated enhancement in tissue adhesion resulted in maximal lap shear strength values of 47.1 ± 3.2 kPa in the HA-Dopa-Polydopa group versus HA-Dopa alone (20.1 ± 2.8 kPa) (Figure 1B). The 100-200 nm sized polydopamine NPs were optimal for maximal increase in tissue adhesiveness of HA-Dopa hydrogels, while variation of nanoparticle concentrations in all NC groups revealed the adhesive strength increase to be concentration-dependent. CONCLUSIONS: We report that these nanocomposites may serve as adhesives for applications in tissue interfacing, such as temporary bio-glues for tissue adhesion. Source of Funding: None Arlington, TX; Dallas, TX; Arlington, TX© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e19-e19 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nikhil Pandey* More articles by this author Andres Urias More articles by this author Valinda Jones More articles by this author Serkan Yaman More articles by this author Amirhossein Hakamivala More articles by this author Boris Rodionov More articles by this author Jun Liao More articles by this author Philippe Zimmern More articles by this author Kytai Nguyen More articles by this author Yi Hong More articles by this author Expand All Advertisement PDF downloadLoading ...