Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
FRONTIERS IN MOLECULAR BIOSCIENCES(2019)
摘要
A variant of 9 degrees N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name "Therminator DNA polymerase" has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.
更多查看译文
关键词
DNA sequencing,xenonucleic acids,aptamers,functionalized DNA,unnatural base pairs
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要