Extracellular-Controlled Separation And 3d Cell Culture By Using Opto-Electrokinetic

Yang Liu,Rong Tan,Yajing Shen

2018 IEEE 8TH ANNUAL INTERNATIONAL CONFERENCE ON CYBER TECHNOLOGY IN AUTOMATION, CONTROL, AND INTELLIGENT SYSTEMS (IEEE-CYBER)（2018）

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摘要

We report a feasible opto-electrokinetic approach to realize rapidly controllable single cell screening and isolating culture in the extracellular environment. A nonuniform AC electric filed makes cells separate and sort by size, shape and viability. The DC electric fields-based dynamically forms the alginate hydrogel in 3D on the top of the organic photoconductive material titanium oxide phthalocyanine. The trapped cells can be cultured in the 3D alginate hydrogel pattern.

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关键词

trapped cells,extracellular-controlled separation,3D cell culture,feasible opto-electrokinetic approach,rapidly controllable single cell screening,isolating culture,extracellular environment,nonuniform AC electric,DC electric fields-based,organic photoconductive material titanium oxide phthalocyanine

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