Liver Total Glycogen, Mitochondrial Protein and CoQ10 Analysis in CoQ10 Supplemented Broiler Chicken

A Pilot study was conducted to find out the level of CoQ10 inclusion in broiler diet to bring about better performance and conducted during the period from November to December. Fifty numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to five groups of ten chicks each based on the body weight. The treatments include, 20mg, 40mg, 60mg, 80mg, 100mg of CoQ10/kg diet. Based on the results of FCR in pilot study experiment II was conducted with Two hundred and forty (240) numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to 5 groups 6 replicates of eight chicks each based on the body weight. The treatments were, Basal diet without CoQ10 supplementation, Low energy diet without CoQ10 supplementation, Low energy diet with 20mg of CoQ10/kg diet, Low energy diet with 40mg of CoQ10/kg diet, Low energy diet with 60mg of CoQ10 /kg diet. Glycogen content of the liver was estimated as per the method of Seifter et al., (1949). Hepatic mitochondria were obtained by differential centrifugation as outlined by Cawthon et al., (1999). Mitochondrial protein concentration was estimated as per the method of Lowry et al., (1971). The CoQ10 content of liver was extracted as per the method of (Barros et al., 2011) and liver CoQ10 level was estimated as per the method of (Ioana et al., 2009). The mean liver glycogen (µg/g) was 152.66, 167.55, 169.14, 171.13 in the treatment groups T2 to T5 as compared with 158.45 in the control group of broiler chickens. The mean liver glycogen was significantly (PË‚0.05) highest in the Co Q10 supplemented groupof birds (T3, T4 and T5) in comparison to control and T2 group. There was no significant difference in mean liver glycogen among the treatment groups T4 and T5. The mean liver glycogen was significantly (PË‚0.05) lowest in the control and T2 group of birds. The mean liver mitochondrial protein concentration (µg/g) was 15.90, 24.70, 22.00 and 23.20 in the treatment groups T2 to T5 as compared with 19.70 in the control group of broiler chickens. The mean liver mitochondrial protein concentration was significantly (PË‚0.05) highest in the CoQ10 supplemented group of birds (T3, T4 and T5) in comparison to control and T2 groups. The mean liver CoQ10 (mg/kg) was 49.26, 54.53, 55.75 and 55.74 in the treatment groups as compared with 53.83 in the control group of chicken.