CAR T-cells’ Interaction with Artificial Antigen Presenting Cell Surface

Adoptive Cell Transfer (ACT) using Chimeric Antigen Receptor (CAR) T-cells shows promising results in treating metastatic melanoma and B cell malignancies. However, the detailed mechanism of CAR T-cell’s interaction with tumors is still not well-understood. In this work, we created an artificial antigen presenting cell (APC) surface for 2nd generation (CD3 and CD28 signaling domains) CD19 and GD2 CAR T-cells and studied the interaction of CAR T-cells in terms of synapse formation. We created circular patterns of antibodies against CAR T-cells using micro-contact printing and measured the level of activation and degranulation in both CAR T-cells by quantifying the intensity of the phosphorylated CD3ζ chain (p-CD3ζ) and the Lysosome-Associated Membrane Protein 1 (LAMP-1) respectively. The results show that we can study the interaction of CAR T cell with APC surface in a reproducible manner and that different CAR T cells have a different level of activation and degranulation. Our findings suggest that the ar...