Exploring the relationship between cytoplasmic ion content variation and multidrug resistance in cancer cells via ion-release based impedance spectroscopy

•A new version of ion release-based impedance spectroscopy is implemented.•Average cytoplasmic ion contents are determined at single cell level.•Drug resistant cancer cells possess higher ion concentration than wild types.•Ion content increase: 1.8-fold for high-level lab & 1.2-fold for clinically-relevant models.•Potential usage in clinically-relevant models with high sensitivity measurements.