Improvement of in vitro donor plant competence to increase de novo shoot organogenesis in rose genotypes

•Various components influence the in vitro growth and physiology of Rose.•Impact of sugar (type and concentration) on the in vitro Rose development and de novo shoot organogenesis.•High variability in growth and development between Rose genotypes.•Sorbitol detrimental to growth and organogenesis in Rose tested genotypes.•Protocol to induce Rose de novo shoot organogenesis.