Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability

•Phanerochaete chrysosporium cellobiose dehydrogenase was cloned and expressed in S.cerevisiae.•Enzymatic assay in microtiter plates based on 2,6-dichloroindophenol was optimized.•Several mutants of cellobiose dehydrogenase with increased activity were found.•Recombinant cellobiose dehydrogenases were purified and characterized.