Measuring Enzymatic Stability By Isothermal Titration Calorimetry
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(2019)
摘要
This work demonstrates a new method for measuring the stability of enzyme activity by isothermal titration calorimetry (ITC). The peak heat rate observed after a single injection of the substrate solution into an enzyme solution is correlated with enzyme activity. Multiple injections of the substrate into the same enzyme solution over time show the loss of enzyme activity. The assay is autonomous, requiring very little personnel time, and is applicable to most media and enzymes.
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关键词
Environmental Sciences,Issue 145,Industry,Food Industry,Food Technology,Chemistry Techniques,Analytical,Calorimetry,Enzyme Assays,Analytical,Diagnostic and Therapeutic Techniques and Equipment,Investigative Techniques,Technology,Industry,Agriculture,Technology,Industry,and Agriculture,isothermal titration calorimetry,differential scanning calorimetry,lactase,invertase,enzyme immobilization,enzyme activity,calorimetry
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