Vacuum infiltration enhances the Agrobacterium-mediated transient transformation for gene functional analysis in persimmon (Diospyros kaki Thunb.)
Scientia Horticulturae(2019)
Abstract
•A transient transformation system by vacuum infiltration for persimmon was developed.•This system is applicable to different persimmon genotype.•The DkLAR transient silencing leads to significant decrease in PAs levels of persimmon leaves.
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Key words
GFP,GUS,LAR,C-PCNA,J-PCNA,PVNA,PVA,PCA,LB media,CaMV 35S,OCS,NosP-NPTⅡ
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