Abstract 19402: A Novel Mode of Mitochondrial Transplantation: Intracoronary Delivery of Autologous Mitochondria Provides Prolonged Increase in Coronary Blood Flow

Introduction: Autologous mitochondrial transplantation by direct injection has shown to be cardioprotective in ischemic injury. Herein, we investigate a novel mode of mitochondrial transplantation by intracoronary delivery to enable whole organ therapy. Methods: Autologous mitochondria from pectoralis muscle of Yorkshire pigs (N=14) were injected into LCA via angiography catheter. Uptake and distribution was assessed by microscopy and PET CT. Safety of delivery under coronary vasoconstriction and tachycardia was assessed by coinjection with ADH and epinephrine. Coronary patency and flow were assessed by angiography and transonic flow probe. LV function was measured by sonomicrometry and PV catheter. Endothelium and smooth muscle dependent pathways of coronary vasodilation was investigated by using inhibitors of eNOS, adenosine-receptors and cyclooxygenase. Results: Intracoronary injection of mitochondria showed wide and specific distribution throughout the LV with no effect on rhythm, HR, MAP or LVEDP and...