Gene Delivery: Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium (Adv. Mater. 8/2019)

In vitro transcribed messenger RNA is nebulized using poly(beta amino esters) for inhalable delivery to the lung as described by Daniel G. Anderson and co-workers in article number 1805116. Hyperbranched polymers are synthesized, which form stable mRNA-polyplexes that distribute uniformly throughout the lungs of mice and are translated to reporter protein in the lung epithelium.