A New, Rapid, Efficient And Non-Toxic Method For Bacterial Dna Extraction

The extraction of DNA from biological materials is most important initial stage based under all molecular genetic analyses. Efficient and pure DNA extraction is mandatory in order to achive succesful PCR results and accurate molecular genetic evaluations of the results. We invented a new, rapid, efficient and non-toxic method for bacterial DNA extraction from infected host tissue. With this method we successed separate isolation of Helicobacter pylori DNA from patients gastric biopsy specimens. The isolation of DNA from trace organic materials and from trace microbial contaminants becomes the issue. Mostly the infected organic materials taken by biopsies are such miligrams trace materials and are non-reproduceable tissues taken by hard, painful invasiv interventions. Therefore the efficient DNA isolation from such tissues is very important. With this method we successed separate isolation of bacrerial DNA from host biopsy tissues without using toxic hazardeous chemicals. Our metod we tested and found that it is efficient for bacterial DNA extraction from very small miligram amounts (trace) biopsy materials and obtaining bacterial DNA seperate from host DNA. In our this presentation we are aiming to introduce the details of our this new method we have designed, tested, used and achieved extremly efficient PCR results and accurate genotyping evaluations of Helicobacter pylori strains isolated from gastric biopsies. We are going to register patent application and prepare customized analytical kit for this isolation procedure by a new scientific Project.