Chrome Extension
WeChat Mini Program
Use on ChatGLM

Lymphocyte Phenotypic Analysis of Peripheral Blood Mononuclear Cells in Patients with Systemic Mastocytosis

Journal of Allergy and Clinical Immunology(2019)

Cited 0|Views12
No score
Abstract
Mastocytosis is a clonal disorder of aberrant mast cell proliferation and burden. Symptoms are associated with excessive mast cell mediator release. Mast cell mediators have been reported to affect lymphocyte differentiation and function, but lymphocyte subsets have not been characterized in detail and correlated with clinical findings in patients with mastocytosis. Peripheral blood mononuclear cells (PBMCs) from 20 patients with indolent systemic mastocytosis (ISM) were analyzed to quantitate lymphocyte subpopulations by flow cytometry and correlated with clinical parameters of disease. DNA was extracted from patient PBMCs and analyzed to determine the allelic frequency of the mastocytosis-associated KITD816V mutation. The median frequency and absolute cell count of all lymphocyte subsets examined in this cohort were found to be within the normal range of healthy adult individuals. We then stratified the results according to clinical manifestations (autoimmunity, atopic disease, anaphylaxis, cancer, osteoporosis/osteopenia and chronic/recurrent infections) to determine if the lymphocytes subsets correlated any of these manifestations. We observed the median % (p=0.032) and absolute count (p=0.015) of CD19+CD21lowCD38lowB cells were significantly higher in patients with a history of autoimmune disease. Counts of terminally differentiated CD4+ T cells were significantly higher in patients with osteoporosis (p=0.038) or osteopenia (p=0.045). Lymphocyte subsets in the peripheral blood of patients with ISM were in general not significantly different from those of the general population. Extensive lymphocyte phenotyping of PBMCs may, however, be able to help predict or monitor some specific clinical manifestations associated with ISM.
More
Translated text
Key words
Mastocytosis,Mast Cells
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined