Surface Imprinted Ag Decorated Mno2 Thin Film Electrodes For The Synergic Electrochemical Detection Of Bacterial Pathogens

Pathogenic bacterial detection is needful in disease diagnosis as well as in the prevention of bacterial infections. This work proposes the electrochemical detection of pathogenic bacterial cells of Staphylococcus aureus (S. aureus) in a simple and cost-effective manner. We have developed a surface imprinted matrix on silver (Ag) decorated Manganese dioxide (MnO2) thin film coated Fluorine doped Tin oxide (FTO) electrode. The pulsed electrodeposition technique has been employed to develop the Ag decorated MnO2 thin films. The surface imprintation was done by using S. aureus as a model bacterial template, Polyethyleneimine (PEI) as a molecular recognizer and nafion as an additive to stick the imprinted PEI to the surface of the film. In order to find the binding probabilities, docking studies were done between PEI with the bacterial cell wall components. From cyclic voltammetry (CV) analysis, we found the presence and absence of the bacterial cells lead to the variability in the redox peaks corresponds to the redox activity of [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-) has been found. Based on this, Differential Pulse Voltammetry (DPV) analysis was performed with different bacterial concentrations resulted in the detection range of 10(3) to 10(7) CFU/ml. (C) 2019 The Electrochemical Society.