Development of a 450 nm Laser Irradiation Desorption Method for Fast Headspace Solid‐Phase Microextraction of Volatiles from Krill Oil (Euphausia superba)

A laser irradiation desorption (LID) method is developed using a 450 nm blue semiconductor laser and gas chromatography mass spectrometry (GC/MS) for analyzing the flavor of Antarctic krill oil (Euphausia superba). The experimental parameters of LID are optimized by response surface methodology (RSM), and the optima are obtained as follows; laser power (10 W), irradiation time (5 min), and sample volume (3 g). The results show that a total of 41 volatile molecular species are detected, including six alcohols, 10 aldehydes, five ketones, 14 hydrocarbons, and six other compounds. Compared with the conventional heating assisted desorption technique, the LID can release volatile molecular species with higher abundance in a considerably reduced extraction time from 30 to 5 min. Finally, the proposed method is validated in terms of sensitivity, precision, linearity, and recovery, which demonstrate its advantages of high efficiency and simplified procedure. Practical Applications: An LID method is developed using a 450 nm blue semiconductor laser for analyzing the flavor of Antarctic krill oil (E. superba). Compared with the conventional heating assisted desorption technique, the LID can release more volatile molecular species with higher abundance in a considerably shorter extraction time, and the intensive laser power does not induce the autoxidation of polyunsaturated fatty acids. It shows the advantages of a highly efficient, and simplified procedure.