Gene expression pattern of NEDD4-1/2 in the developing inner ear.

Event Abstract Back to Event Gene expression pattern of NEDD4-1/2 in the developing inner ear. Ronald L. Pouyo1*, Amandine Czajkowski1, Laurence Delacroix1, Stephen Freeman1 and Brigitte Malgrange1 1 Neurodevelopmental biology – GIGA Neurosciences. University of Liege, Belgium Protein ubiquitination is an important post-translational modification that is involved in multiple cellular processes such as protein degradation, endocytosis, sorting, trafficking and turnover. A recently identified mutation in human NEDD4L gene, which encodes the E3 ubiquitin-ligase NEDD4-2, has been shown to lead to several abnormalities in patients such as cerebral atrophy, optic atrophy, and hearing impairment. This observation, combined with the fact that NEDD4-2 knockout mice are deaf lead us to investigate the roles of NEDD4-1/2 in the development of the inner ear. The first part of the project focuses on establishing the expression pattern of NEDD4-1/2 mRNAs and proteins in the inner ear by in situ hybridization and immunohistofluorescence, respectively. We show that both NEDD4-1 and NEDD4-2 are dynamically expressed in the embryonic cochlea, with transcripts present in the mechanosensory hair cells, the greater epithelium ridge, spiral ganglion neurons and the stria vascularis. We notice that while NEDD4-1 expression remains in these structures at postnatal stages, NEDD4-2 expression is significantly reduced. This NEDD4-2 expression profile suggest a role of NEDD4-2 in cochlear cell proliferation, differentiation and patterning. Functional and histological studies are ongoing on NEDD4-2 knockout animals in order to decipher its role on hearing and cochlear development. Keywords: ubiquitination., Gene Expression, Nedd4, Cochlea, Hair Cells, Auditory Conference: 12th National Congress of the Belgian Society for Neuroscience, Gent, Belgium, 22 May - 22 May, 2017. Presentation Type: Poster Presentation Topic: Development Citation: Pouyo RL, Czajkowski A, Delacroix L, Freeman S and Malgrange B (2019). Gene expression pattern of NEDD4-1/2 in the developing inner ear.. Front. Neurosci. Conference Abstract: 12th National Congress of the Belgian Society for Neuroscience. doi: 10.3389/conf.fnins.2017.94.00092 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Apr 2017; Published Online: 25 Jan 2019. * Correspondence: Mr. Ronald L Pouyo, Neurodevelopmental biology – GIGA Neurosciences. University of Liege, Liège, Belgium, lrpouyo@student.ulg.ac.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ronald L Pouyo Amandine Czajkowski Laurence Delacroix Stephen Freeman Brigitte Malgrange Google Ronald L Pouyo Amandine Czajkowski Laurence Delacroix Stephen Freeman Brigitte Malgrange Google Scholar Ronald L Pouyo Amandine Czajkowski Laurence Delacroix Stephen Freeman Brigitte Malgrange PubMed Ronald L Pouyo Amandine Czajkowski Laurence Delacroix Stephen Freeman Brigitte Malgrange Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.