Determination of Ellagic Acid in Wine by Solid-Phase Extraction–Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry

The method for the determination of ellagic acid in wine by solid-phase extraction–ultra-high performance liquid chromatography–tandem mass spectrometry (SPE-UPLC-MS/MS) using electrospray ion source (ESI) multiple reaction monitoring (MRM) mode detection was established. The sample was purified by Oasis HLB solid-phase extraction column and separated on an Acquity UPLC® BEH C18 column (2.1 mm × 100 mm 1.7 μm) with a mobile phase of 0.1% formic acid methanol–0.1% formic acid aqueous solution. Under the best detection conditions, ellagic acid within the range of 0.01~1 mg/L had a good linear relationship with a correlation coefficient of 0.9993. The detection limit (LOD) and quantitative limit (LOQ) were 3 and 10 μg/L, respectively. The recovery was 90.8~116% with the relative standard deviation (RSD) of 3.6~9.4%. The results indicate that the method is simple, fast, sensitive, and reproducible and can be used for the determination of ellagic acid in wine.