Protective effect of andrographolides against docetaxel-induced cognitive impairment in rats bearing mammary tumours

Event Abstract Back to Event Protective effect of andrographolides against docetaxel-induced cognitive impairment in rats bearing mammary tumours Nasir I. Khatab1, Dahiru Sani1, Brian P. Kirby2, Norhafizah, Muhtarrudin1, Ho G. Fuang3, Johnson Stanslas1* and Hamidon Basri1* 1 Putra Malaysia University, Malaysia 2 Royal College of Surgeons in Ireland, Ireland 3 University Malaya Medical Centre, Malaysia Background Andrographolide (AGP) and 14-deoxy-11,12-didehydroandrographolide (DDAG) are two main diterpenoid constituents of Andrographis paniculata, which have shown several therapeutic effects. The aim of this study was to examine the protective effects of AGP and DDAG against cognitive impairment (CICI) caused by chemotherapeutic agent, docetaxel (DTX), on rats with mammary tumours. Methods A two-day Morris Water Maze (MWM) protocol, which involved training with a visible platform on day one followed by testing with a hidden platform on day two, was performed. Ten groups of female Sprague Dawley rats were used. The rats were injected with LA7 cells into the mammary gland pad to form tumours and further divided into groups of normal control (NC), cancer control (CC), treatment with DTX (single dose 5 mg/kg i.p.), three doses (0.25, 0.50 and 1.0 mg/kg) of AGP or DDAG and a vehicle. Subsequently, AGP, DDAG or vehicle was administered intraperitoneally for seven consecutive days followed by DTX injection after day one of visible platform trial. Levels of pro-inflammatory cytokine interleukin IL-1, lipid peroxidation marker (thiobarbituric acid-reactive substances (TBARS) and reactive oxygen species (ROS)) were measured. Results After 24 hours of DTX injection, the escape latency of DTX had significantly (p<0.001) increased to 58.1 sec compared to 24.7 sec in CC. AGP and DDAG significantly (p<0.001) reduced this DTX-induced latency to about 10-16 sec. AGP and DDAG significantly (p<0.001) reduced the elevated IL-1β (78-91%), IL-6 (55-75%), TNF-α (63-73%), TBARS (52-83%) and ROS (22-50%) levels when compared to DTX. AGP and DDAG did not affect the anti-cancer activity of DTX. Conclusion AGP and DDAG, if given prior to DTX, may be protective against the chemotherapy-induced cognitive impairment. Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Acknowledgements The authors acknowledge the Ministry of Agriculture and Agro-Based Industry Malaysia for funding this research (NRGS Grants NH1014D025). Keywords: AGP, DDAG, DOCETAXEL, Morris water maze, cognitive impairment Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Neurodegenerative diseases Citation: Khatab NI, Sani D, Kirby BP, Muhtarrudin N, Fuang HG, Stanslas J and Basri H (2019). Protective effect of andrographolides against docetaxel-induced cognitive impairment in rats bearing mammary tumours. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00134 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Prof. Johnson Stanslas, Putra Malaysia University, Seri Kembangan, Malaysia, jstanslas@yahoo.co.uk Prof. Hamidon Basri, Putra Malaysia University, Seri Kembangan, Malaysia, hamidonbasri@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nasir I Khatab Dahiru Sani Brian P Kirby Norhafizah, Muhtarrudin Ho G Fuang Johnson Stanslas Hamidon Basri Google Nasir I Khatab Dahiru Sani Brian P Kirby Norhafizah, Muhtarrudin Ho G Fuang Johnson Stanslas Hamidon Basri Google Scholar Nasir I Khatab Dahiru Sani Brian P Kirby Norhafizah, Muhtarrudin Ho G Fuang Johnson Stanslas Hamidon Basri PubMed Nasir I Khatab Dahiru Sani Brian P Kirby Norhafizah, Muhtarrudin Ho G Fuang Johnson Stanslas Hamidon Basri Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.