Study on Dormancy Mechanism and Breaking Dormancy Method of <i>Viburnum sargentii</i> Seeds

Underuntreated conditions, the germination rate of Viburnum sargentii seedsis very low. By exploring the relationship between the dynamic changes ofphysicochemical indicators and endogenous hormones and seed germination duringthe dormancy and germination of Viburnum sargentii seeds,the mechanism of seed germination of Viburnum sargentii wasdetermined, which provided a theoretical basis for its extensive promotion anddevelopment. The contents of soluble sugar and soluble starch were determinedby anthrone colorimetry. The soluble protein was determinedby Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase(PPO) was determined by NBT reduction method. The peroxidase (POD) wasdetermined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under naturalconditions, the seeds of Viburnum sargentii aredifficult to germinate. 2) Under sand storage for 8 months, the germinationrate of untreated seeds was 33%. Puncture and peeling treatment couldsignificantly increase the germination rate, and the germination rate of seedstreated with puncture was 92%, and that treated with peel was 98%. 3) Seedgermination was accompanied by the decrease in macromolecular substances suchas soluble sugar, soluble starch, and soluble protein. 4) The dynamic changesof hormones during seed germination conform to the hypothesis of “threefactors”. 5) The treatment of puncture and peeling increased the content ofendogenous hormones promoting germination, decreased the endogenous hormonesinhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA orGA/ABA. The seeds of Viburnum sargentii haveobvious dormancy characteristics. Under the condition of sand storage, bothpricking and peeling treatment can effectively promote the process of breakingdormancy, and the effect of peeling treatment is better.