Thymoquinone-loaded nanostructured lipid carrier improved spatial learning, memory and exploratory behaviour in Alzheimer’s disease animal model

Event Abstract Back to Event Thymoquinone-loaded nanostructured lipid carrier improved spatial learning, memory and exploratory behaviour in Alzheimer’s disease animal model Fatin Hannani Zakarial Ansar1, Latifah Saiful Yazan2*, Ng Wei Keat1, How Chee Wun3, Rasedee Abdullah3, Mohamad Aris Mohd Moklas4 and Cheah Pike See4 1 Laboratory of Molecular Biomedicine, Institute of Bioscience, University Putra Malaysia, Malaysia 2 Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Malaysia 3 Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, University Putra Malaysia, Universiti Putra Malaysia, Malaysia 4 Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia Background Alzheimer’s disease (AD) is a devastating neurodegenerative disease with progressive and irreversible damage to thinking, memory and language. Its viable cure remains unavailable. Research on bioactive compounds from natural products with neuroprotective effects and low toxicity is on demand. Thymoquinone (TQ), a compound from Nigella sativa possesses remarkable antioxidant properties is able to protect cells from degradation. However, TQ has low solubility in blood and poor oral bioavailability. Hence, nanostructured lipid carrier (NLC) has been developed as a drug delivery vehicle to overcome the limitations (herein referred as TQ-NLC). This study aimed to evaluate the neuroprotective effects of TQ-NLC as a potential candidate drug for the management of AD in vivo. Methods Sprague Dawley rats were fed with high fat cholesterol diet as a model of Alzheimer’s disease, treated with 12.5, 25 and 50mg/kg of TQ-NLC and TQ, were subjected to spatial learning and memory using Morris water maze. Lipid profile level was also determined using an automated hematology analyzer. Results The animals treated with 25mg/kg of TQ-NLC showed an increase in the time spent at the targeted quadrant and reduced in the total cholesterol compared to the negative control (untreated) (p<0.05). In addition, the animals treated with 25mg/kg of TQ-NLC showed shorter escape latency in comparison to the negative control (untreated) but it was not statistically significant. Conclusion Treatment with TQ-NLC improved spatial learning and memory of the animals and their cholesterol level. Keywords: Alzheimer’s disease, Thymoquinone, NLC (nanostructured lipid carriers), TQ-NLC, high fat cholesterol diet Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Neurodegenerative diseases Citation: Zakarial Ansar F, Saiful Yazan L, Wei Keat N, Chee Wun H, Abdullah R, Mohd Moklas M and Pike See C (2019). Thymoquinone-loaded nanostructured lipid carrier improved spatial learning, memory and exploratory behaviour in Alzheimer’s disease animal model. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00060 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Latifah Saiful Yazan, Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Kuala Lumpur, Malaysia, latifahsy@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fatin Hannani Zakarial Ansar Latifah Saiful Yazan Ng Wei Keat How Chee Wun Rasedee Abdullah Mohamad Aris Mohd Moklas Cheah Pike See Google Fatin Hannani Zakarial Ansar Latifah Saiful Yazan Ng Wei Keat How Chee Wun Rasedee Abdullah Mohamad Aris Mohd Moklas Cheah Pike See Google Scholar Fatin Hannani Zakarial Ansar Latifah Saiful Yazan Ng Wei Keat How Chee Wun Rasedee Abdullah Mohamad Aris Mohd Moklas Cheah Pike See PubMed Fatin Hannani Zakarial Ansar Latifah Saiful Yazan Ng Wei Keat How Chee Wun Rasedee Abdullah Mohamad Aris Mohd Moklas Cheah Pike See Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.