Multi-Functional Neural Probes For Pharmacological And Optogenetic Manipulation And Detection Of Neurotransmitter Release.

The ability to monitor neurotransmitter release in freely behaving animals is key to understanding neuronal processes underlying complex behaviors. Commonly used methods either offer rapid measurements of a single analyte (e.g., fast-scan cyclic voltammetry) or provide multiple analyte measurement with insufficient temporal resolution (microdialysis). However, behaviors are controlled by neuronal networks employing multiple neurotransmitters and neuromodulators. Efforts to understand how neurons within these networks interact to control behavior will be greatly facilitated by a means with which to both modulate and measure multiple neuroactive molecules simultaneously and in near-real time. To this end, we are developing MEA enzymatic biosensors with built-in microfluidics and optical guides for pharmacological and optogenetic control of transmitter release in brain tissue.