A new approach for downstream purification of rhamnolipid biosurfactants
Food and Bioproducts Processing（2019）
Abstract
•Excess oil in the harvested broth makes downstream processing challenging.•Biopolymer removal step using ethanol was effective in improving the recovery.•Rhamnolipids can be back extracted to aqueous phase from solvent with 97% recovery.•Calcium precipitation can selectively remove impurities and improve product purity.
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Key words
Downstream processing,Rhamnolipids,Solvent extraction,Calcium precipitation,Alcohol precipitation,Fermentation
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