Chemoprotective Efficacy Of Salvianolic Acid B Via Triggering Apoptosis In Mcf-7 Human Breast Cancer Cells

Background and Objective: Salvianolic acid B (SB) is one of the active components of a popular Chinese herb called Salvia miltiorrhiza, which possess numerous biological activities including anti-tumor. Current study was framed to evaluate the chemoprotective efficiacy of SB on human breast cancer MCF-7 cells. Materials and Methods: MCF-7 cells were exposed to dimethyl sulfoxide (DMSO-Control) as well as treated with an increased concentration of SB (25, 50, 100 and 200 mu m) to explore the anti-proliferative (Cell viability), pro-oxidative and pro-apoptotic activities. Statistical analysis was carried out by student t-test using SPSS software. Results: MCF-7 cells treated with an increased concentration of SB after 24 and 48 h showed a sharp decline in the count of viable MCF-7cells and thus showcased its potent anti-proliferative and toxicity property. A pronounced increase in ROS generation was noted in MCF-7 cells co-cultured with SB in dose and time-dependent fashion. Likewise, DNA fragmentation and apoptotic markers like caspase 3 and 9 were also significantly increased upon treatment with SB in a dose-dependent manner to display its pro-oxidative and pro-apoptotic activities. Moreover, the protein expression of Bcl2 and Bax were notably downregulated and upregulated respectively after administration with increased concentration of SB than DMSO treated MCF-7 cells. Conclusion: Taking together, that increased concentration of SB could show better chemoprotective activity on human breast cancer MCF-7 cells by exhibiting anti-proliferative, pro-oxidative and pro-apoptotic activities in a dose-dependent manner.