Global analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing

Michael B. Wolfe,Aaron C. Goldstrohm,Peter L. Freddolino

Methods（2019）

引用 5|浏览8

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摘要

•Metabolically labeled RNA is used to illuminate RNA decay rates.•Several variations in label chemistry and experimental design exist.•Proper data analysis is critical for transcriptome-wide RNA decay interpretation.•Modularity in RNA decay experimental methods allows for custom designs.

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关键词

RNA decay,Metabolic labeling,High-throughput sequencing,4sU,EU,BrU

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