Efficacy of Clostridium butyricum as probiotic feed additive against experimental Salmonella Typhimurium infection in pigs

•Successful inoculation, resulting in subclinical S. typhimurium infection.•C. butyricum did not significantly reduce fecal excretion and serological response.•C. butyricum did not significantly reduce intestinal carriage.•C. butyricum did not significantly reduce prevalence in the ileocecal lymph nodes.•Further research is needed to investigate the results under field conditions.