Fluorescence anisotropy of fluorescein derivative varies according to pH: Lessons for binding studies

Journal of Photochemistry and Photobiology A: Chemistry(2019)

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Abstract
•Fluorescence emission of a fluorescein derivative decreased in acid pH.•Fluorescence anisotropy of fluorescein derivative increased in acid pH.•The monoanionic species of fluorescein derivative predominates under physiological pH.•A constant fluorescence emission of fluorescein derivative is a prerequisite for binding studies.
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Key words
Fluorescence spectroscopy,Fluorescein derivative,6-FAM,Anisotropy,dsDNA
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