Fluorescence anisotropy of fluorescein derivative varies according to pH: Lessons for binding studies

Nathália S.S. Castro,Isabel Moura,Marta S.P. Carepo,César A.T. Laia

Journal of Photochemistry and Photobiology A: Chemistry（2019）

引用 3|浏览14

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摘要

•Fluorescence emission of a fluorescein derivative decreased in acid pH.•Fluorescence anisotropy of fluorescein derivative increased in acid pH.•The monoanionic species of fluorescein derivative predominates under physiological pH.•A constant fluorescence emission of fluorescein derivative is a prerequisite for binding studies.

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关键词

Fluorescence spectroscopy,Fluorescein derivative,6-FAM,Anisotropy,dsDNA

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