Synthesis, Spectral Characterization, Docking Analysis, DNA Binding/Cleavage, Antimicrobial and Cytotoxic Activity of New Dimeric Antipyrine-Schiff Base Metal Complexes

In the present study, a new series of novel Schiff base ligand derived from (furan-2-carbaldehyde and 4-aminoantipyrine) and dimeric metal(II) complexes with the composition of [MLCl2]2, where M=Cu(II), Co(II), Ni(II) and Zn(II) have been synthesized and characterized by elemental analysis, magnetic susceptibility, molar conductivity measurements, FT-IR, UV-visible, 1 H NMR, 13 C NMR, EPR, MS (ESI), XRD, SEM and EDX studies.The spectral data recommend that the dimeric metal complexes embrace octahedral geometry around the central metal ions.The DMF solutions of the dimeric metal complexes demonstrate the lower molar conductance values which might be due to the non-electrolytic nature of the complexes (5.67-13.24Ω -1 mol -1 cm -2 ).The biological studies involved are DNA interaction (binding and damage) antimicrobial, anti-proliferative and molecular docking.DNA interaction of these complexes carried out with using calf thymus DNA (CT-DNA) by electronic absorption titration, viscometric measurement which revealed that the synthesized dimeric complexes interact with DNA through intercalative binding mode.A gel electro-phoresis assay testifies the ability of complexes to cleave supercoiled pUC19 DNA in the presence of hydrogen peroxide as an activator.The synthesized compounds were initiated from their biological perspective.The antimicrobial assay indicates that dimeric complexes are good antimicrobial agents.Besides, in vitro antiproliferative activity of dimeric complexes were investigated on MCF-7, Hep G2, HBL-100 cell lines using an MTT assay.In addition, the molecular docking study was accomplished to considerate the nature of binding of the synthesized compounds with protein and DNA.