Intracytoplasmic sperm injection (ICSI) of prepubertal goat oocytes using fresh and frozen-thawed semen

Irene Menéndez-Blanco,Maria-Gracia Catala,Montserrat Roura,Sandra Soto-Heras,Anna-Rita Piras,Dolors Izquierdo,Maria-Teresa Paramio

Small Ruminant Research（2019）

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摘要

•In vitro Embryo production is an ART with high output variability.•Intracytoplasmic Sperm Injection (ICSI) can help to avoid the sperm effect.•We have studied the use of fresh and frozen-thawed in goat oocytes fertilized by ICSI.•Not differences in blastocyst production were found.

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关键词

Ruminants,Embryos,Sperm analysis

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