Seasonal differences in drivers of species richness of waders in inland wetlands of La Mancha Húmeda Biosphere Reserve

Temporary inland wetlands are among the most threatened ecosystems worldwide, and recognition of the factors that determine species richness in different seasons is key for developing conservation plans for these systems. La Mancha Humeda Biosphere Reserve in central Spain has many inland wetlands of this type, but the driving ecological processes of species richness are poorly understood. This study examines the association of landscape and local variables with species richness patterns of inland wetlands of La Mancha Humeda Biosphere Reserve during winter and the breeding season. The number of lakes in several increasing radii was a proxy of connectivity, and maximum flooded surface and shoreline length were surrogates of the species-area relationship. Other landscape and local habitat variables, such as hydroperiod (length of inundation period), distance from human settlements, shoreline development index, vegetation surface cover, average lake depth, number of islands and surface area of islands were also analysed. Hierarchical partitioning analysis was used to evaluate the contribution of the environmental variables to explain the species richness of waders. Species richness (26 species, four threatened in Europe) had different associations with the variables during the wintering and breeding seasons. The richness of breeding and wintering species was positively associated with hydroperiod and maximum flooded surface area respectively. No variable measured was negatively associated with species richness in any season. Hydroperiod and flooded surface area are altered directly by human activities. Water extraction for irrigation reduces hydroperiod in some wetlands, whereas wastewater input extends hydroperiod in others, promoting wader concentrations in lakes flooded in summer and potentially favouring botulism outbreaks. In addition, the lack of protection and management on most of the lakes in La Mancha Humeda Biosphere Reserve also favours encroachment of agriculture, destroying and degrading habitat for wintering and breeding waders.