Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats

Abstract OBJECTIVE To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action. METHODS In the study, 18 F-2-fluoro-deoxy-D-glucose positron emission tomography ( 18 F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and 18 F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0. RESULTS After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (all P P P CONCLUTION TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.