Salmonella enterica serovar Miami Possessing Both Virulence and Extended-Spectrum β-Lactamase Resistant Genes Isolated from Diarrhoeic Piglets of North East India (Mizoram)

The present study report findings of an acute gastroenteritis outbreak in an unorganized pig farm in the tribal area of North East India (Mizoram).Faecal samples were collected from 12 pigs including 2severely diarrhoeic piglets.A total of 25 E. coli were isolated, all of which were found to be negative for any putative virulence genes of STEC, ETEC, EHEC and EPEC pathotypes.However, no samples were found to be positive for Clostridium spp, viral (Rotavirus, Picobirna virus) and parasitic pathogens (eggs or larvae) under the study.Salmonella isolates (n=2) were isolated and confirmed as Salmonella enterica serovar Miami (9,12:a:1,5).PCR amplification of the genes in study showed present of enterotoxin (stn), invasive (invA) as well as ESBL bla TEM-1 and bla CMY-2 genes.Analysis of the strains by RAPD-PCR indicates that the strains were genetically different from other Salmonellae.The resistance trait from the isolates could not be transferred to the recipient host by the conjugation method.The isolated Salmonella strains were found to be multi drug resistant (MDR) strains.The present study report the isolation of Salmonella Miami strains possessing both virulence and ESBL genes, from severely diarrhoeic piglets in the tribal area of North East India (Mizoram).