bFGF and Poly‐RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation
ADVANCED MATERIALS INTERFACES(2018)
Abstract
Biointerface design is widely used to functionalize biomaterials with controllable physicochemical properties. Functionalized biointerface provides a versatile platform to connect biological entities and nonbiogenic materials. Existing nanofabrication approaches to create such a nanostructured biointerface involve in low stability of the functionalized nanolayer and simple functionalities that limit its applicability. Here, a stable nanolayered synthetic polypeptide (poly[LA-co-(Glc-alt-Lys)] and modified with arginine-glycine-aspartic acid, PRGD)/basic fibroblast growth factor (bFGF) biointerface is created via structural matching, charge interaction, and hydrogen bonding. The cooperative effect of the PRGD/bFGF biointerface shows multiple functionalities in promoting stem cell adhesion by 33% increase in cell adhesion to poly(d,l-lactic acid) substrate as compared to experiments on bare substrate as a control. Moreover, the biointerface enhances proliferation by 40% in cell density, potential differentiation by 62%, and gene expression by 40 and 80% respectively as compared to the control samples. The fabricated biointerface may have applications in nerve regeneration, tissue repair, and stem cell-based therapy.
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Key words
biointerface,cell proliferation,cooperative effect,growth factors,stem cell differentiation
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